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. 2008 Sep 8;155(6):934–944. doi: 10.1038/bjp.2008.323

Figure 6.

Figure 6

Magnified images of the effects of PTX-2 (200 nM) on the actin cytoskeleton and morphology of Clone 9 cells. Images correspond to × 2 digital zooms. Panels (a) and (c) are fluorescence and transmission photographs of the control cells, respectively. Panels (b) and (d) are from cells treated with 200 nM PTX-2. Arrows point to differences on the F-actin distribution between control and treated cells (bundles and dots, respectively). One cell is outlined in controls (c) and in cells incubated with PTX-2 (d) to show morphological changes. Images are representative of three independent experiments. Scale bar=50 μm. (e) F- and G-actin fluorescence measurements for Clone 9 rat hepatocytes, showing the percentage (mean±s.e.mean for n ⩾3 experiments) of fluorescently labeled F- and G-actin in cells treated with PTX-1, PTX-2, PTX-2SA or PTX-11 relative to control cells (100%). (f) Viability (mean±s.e.mean of n ⩾3 experiments) of Clone 9 cells by Alamar Blue fluorescence after incubation with 200 nM PTXs or Lat A, relative to control cells (100%).