Figure 5.

Characterization of non-nitrergic responses in strips showing electric field stimulation (EFS)-induced off-contractions. Strips were incubated with N^G-nitro-L-arginine (L-NNA; 1 mM) and atropine (1 μM) before drug addition. Left panels show quantitative effects and right panels show typical recordings. (a) MRS 2179 (10 μM) significantly reduced both latency and amplitude. (b) NF279 (10 μM) had no effect on EFS-induced responses. (c) α-chymotrypsin (α-CMT; 10 U mL⁻¹) increased the amplitude of off-contractions. ^***P<0.001.