Figure 3.

Comparison of the inhibitory effects of NaHS and ACS6 on tumour necrosis factor-α (TNFα) (0; 10 ng mL⁻¹)-induced O₂^•− formation from pulmonary artery endothelial cells: (a) co-incubated over 16-h incubation period and (b) incubated for 2 h only following 16-h incubation with 10 ng mL⁻¹ TNFα alone. Experimental data were averaged from six independent experiments carried out in different batches of cells. Data were normalized to TNFα responses and the curves were obtained fitting data to the sigmoidal dose–response equation described in Methods section. ^*P<0.01; significantly inhibited compared with TNFα-treated cells (0). ^†P<0.05; significantly greater inhibition than NaHS.