Figure 6.

Immunohistochemical staining for endothelin-1 receptor (a) and western blots for ET_A receptor (ET_A-R; b and c) or ET_B receptor (ET_B-R; b and d) in endothelium-intact rat aorta. Representative sections of endothelin-1 receptor-positive staining is shown in red for aorta obtained from control rats, with and without angiotensin II infusion and for insulin-treated diabetic rats (IT-diabetic) with and without chronic losartan therapy. Calibration bar, 25 μm. Original magnification × 100. Representative western blots of ET_A and ET_B receptors from rat aorta (b) were quantified by scanning densitometry (c and d). For the ET_A receptor, two bands (54 and 39 kDa) corresponding to native and glycosylated forms were detected. Values are mean±s.e.mean from 8 experiments. ^*P<0.05 versus controls. ^#P<0.05 versus IT-diabetic.