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. 2008 Sep 22;155(7):1076–1084. doi: 10.1038/bjp.2008.356

Figure 4.

Figure 4

Effect of mast cell mediators on Jurkat cell activation. Jurkat cells were also incubated with (a) supernatant fluid collected from mast cells treated with MBP (10 μM) and IL-2 release was measured in the supernatant fluid by ELISA (n=3); (b) with TNF-neutralizing antibody (n=3) for 1 h before activation with anti-CD3/anti-CD28 and the addition of mast cells with or without MBP. After 48 h, the supernatant fluid was collected and assayed for IL-2 level by ELISA. (a) *P<0.05 compared with condition 3; (b) @,*P<0.05, @=compared with condition 1, *=compared with condition 3.