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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 18;321(1):188–196. doi: 10.1016/j.ydbio.2008.06.014

Figure 2.

Figure 2

Smo and SmoM2 induced Gli-dependent luciferase activity was inhibited by pertussis toxin (PTX), cholera toxin (CTX), forskolin (FSK), and cyclopamine (CPM). (A) ADP-ribosylation assay of Shh-light cells pre-incubated with or without PTX. This 32P-labeled band (approximately 41 kD) presumably corresponds to a mixture of Gαi2 and Gαi3 (Castro et al., 2001; Xu and Barbieri, 1995). See Materials and methods for details of the assay. (B) The reporter assay results. Shh-light cells, which contained a stably integrated Gli-dependent firefly luciferase reporter and a Renillar luciferase reporter control, were infected with a retrovirus containing Smo and SmoM2 cDNAs. After being treated with the indicated drugs for 36–48 hrs, the cells were lysed, and firefly and Renilla luciferase activities were measured. The results shown were obtained from three independent experiments.