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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Biochemistry. 2007 Mar 27;46(16):4861–4875. doi: 10.1021/bi062169g

Figure 2.

Figure 2

Kinetic and thermodynamic framework for the ribozyme reaction. (A) Definition of rate and equilibrium constants used herein. Association rate constants (k1, k4, k7 and k8) are reported in M-1 min−1; all other rate constants are in units of min−1. The internal and external equilibrium constants are unitless and describe conversion of substrates to products. Frameworks are defined at 30 °C, 10 mM MgCl2 and pH 6.2. (B) Kinetic and thermodynamic framework for the L-21 ScaI ribozyme with CCUCdTA bound. This framework was adapted from (5). Rate constants for dissociation of CCUCdTA and CCUCdT were determined herein for direct comparison with the L-16 ScaI reactions. (C) Kinetic and thermodynamic framework for the L-16 ScaI ribozyme with CCUCdTA bound. (D) Kinetic and thermodynamic framework for the L-16 ScaI ribozyme with CCUCdTAAACC bound.