. Author manuscript; available in PMC: 2008 Dec 8.

Published in final edited form as: Biochemistry. 2007 Mar 27;46(16):4861–4875. doi: 10.1021/bi062169g

Table 4.

Binding of the +1 U•U Mismatched Substrate

CCUCdT-	Δ ΔG_bp (kcal/mol)		k_c (min⁻¹)	K_tert
	−G	+G
-AAACC	−5.4^a	−5.4	0.039	23
-UAACC	−3.9	−3.0	0.041	230

All experiments were performed at 30 °C and pH 7.2. All substrates contained a 2′-deoxyribose substitution at the cleavage site (position –1) to prevent cleavage. K_tert values were obtained by comparison of docked and undocked substrates as described in the Materials and Methods, with oligonucleotide substrates undocked by the introduction of a 2′-methoxyribose substitution at position –3 (26, 35, 55). Δ ΔG_bp values were obtained by comparison of the affinity of the CCUCdTAAACC or CCUCdTUAACC with CCUCdTA.

Value from Table 1 included for comparison.