Figure 2. Hairpin formation defect of coding flank sensitive RAG1 mutant can be rescued by oligonucleotide substrate that contains mismatched base pairs.

(A) Oligonucleotide substrates containing matched (M) or mismatched (MM) base pairs as depicted on the left. Triangles above gel indicate increasing incubation times: 5, 10, 20, 40, and 80 min. Reaction products are depicted on the right (from top to bottom): uncleaved substrates, hairpins, and nicks.

(B) Quantitative analysis of gel shown in (A). Reactions performed with matched substrates are shown in solid lines and mismatched substrates in dashed lines.