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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Gastroenterology. 2008 Jun 25;135(4):1344–1357. doi: 10.1053/j.gastro.2008.06.048

Figure 3.

Figure 3

Alteration of the levels of oxidatively-modified proteins in mouse livers following I/R injury. (Left panel) Equal amounts of mitochondrial proteins (20 μg/well) were isolated from sham-control or I/R injured mouse livers (2-h reperfusion) with and without MnTMPyP and analyzed by 12% SDS-PAGE subjected to Coomassie blue staining. (Right panel) Biotin-NM labeled oxidized- or S-nitrosylated-mitochondrial proteins (20 μg/well) were separated on 12 % SDS-PAGE, transferred to a PVDF-Immobilon membrane, and subjected to immunoblot analysis using HRP-conjugated monoclonal antibody against biotin (MAb-biotin-HRP). This figure represents a typical result from two separate experiments.