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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Biochemistry. 2007 Sep 28;46(42):11930–11937. doi: 10.1021/bi700932q

Figure 3.

Figure 3

Effect of pH on PvdA activity. The rate of NADPH oxidation was measured in the presence of ornithine as a decrease in absorbance at 366 nm using 100 mM potassium phosphate at pH 6.0 – 8.0 (filled circles); 33 mM potassium phosphate, 33 mM Tris-SO4, 33 mM glycine at pH 6.0 – 10.0 (open circles); 100 mM Tris-HCl at pH 7.0-9.0 (filled triangles); and 100 mM glycine at pH 9.0 – 10.0 (open triangles). The rate of NADPH oxidation was measured in the absence of ornithine using 100 mM potassium phosphate at pH 6.0 – 8.0 (filled squares), 100 mM Tris-HCl at pH 7.0 – 9.0 (open squares), and 100 mM glycine at pH 9.0 – 10.0 (filled diamonds).