Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Pharmacol. 2008 May 9;74(2):423–431. doi: 10.1124/mol.107.044651

Figure 7. Applications of the pdr1DBD-repressor fusions: sensitive detection of etoposide hypersensitivity in yeast deletion strains.

Figure 7

The pdr1DBD-fusion constructs enhance the sensitivity of various deletion strains to etoposide. Cells carrying a pYX empty vector, pdr1DBD-SIN3, pdr1DBD-CYC8, or Δpdr1::pdr1DBD-CYC8 were tested for sensitivity to etoposide. The strains used were wild type BY4741, BY4741 Δmus81, BY4741 Δctf8, or BY4741 Δsae2. Enhanced sensitivity for a fusion construct is noted by comparing wild type with the three mutants at a fixed etoposide concentration. For example, at 50 μg/ml etoposide, enhanced sensitivity of all three mutants is seen with Δpdr1::pdr1DBD-CYC8. Enhanced sensitivity of the Δctf8 strain is also seen with pdr1DBD-CYC8. At 100 μg/ml etoposide, enhanced sensitivity of the Δctf8 and Δsae2 strains can be clearly discerned.