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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Cancer Cell. 2008 Jul 8;14(1):68–78. doi: 10.1016/j.ccr.2008.05.017

Figure 1. Dimerization of Fbx4 occurs through its N-terminus.

Figure 1

A and B. Lysates were prepared from 293T cells transfected with vectors encoding human wild type Myc-tagged Fbx4 and the indicated Flag-Fbx4 mutant constructs. Protein complexes were isolated by M2 affinity chromatography and detected by immunoblot with a Fbx4 antibody. C. 293T cells were co-transfected with Fbx4 constructs along with cyclin D1, CDK4 and αB-crystallin. 24hrs post-transfection cells were treated with MG132 for 6 hrs followed by cyclin D1 immunoprecipitation. Cyclin D1 was detected with the 13G11 monoclonal antibody.