Table 1. Analysis of Fbx4 mutations in esophageal tumors.
Full-length cDNA encoding Fbx4, cyclin D1 and αB-crystallin were generated by first-strand RT-PCR using oligo dT primer followed by PCR using specific primers. 116 totals samples were sequenced in both directions. Cyclin D1 protein expression was analyzed by immunohistochemistry. ESCC-esophageal squamous cell carcinoma, EAC-esophageal adenocarcinoma. N/A indicates that paraffin embedded tumor tissue was not available for IHC analysis of cyclin D1 accumulation.
| tumor # | Fbx4 | cyclin D1 | CryAB | cyclin D1 protein levels |
|---|---|---|---|---|
| 1 ESCC | L23Q | wt | wt | na |
| 4 EAC | P13S | wt | wt | na |
| 7 ESCC | S12L | wt | wt | +++ |
| 8 ESCC | L23Q | wt | wt | +++ |
| 9 ESCC | wt | wt | wt | + |
| 10 ESCC | wt | wt | wt | +++ |
| 11 ESCC | wt | wt | wt | ++ |
| 12 ESCC | wt | wt | wt | na |
| 14 ESCC | wt | wt | wt | na |
| 15 ESCC | S8R | wt | wt | +++ |
| 16 ESCC | wt | wt | wt | na |
| 17 ESCC | wt | wt | wt | na |
| 18 ESCC | wt | wt | wt | + |
| 19 ESCC | P76T | wt | wt | +++ |
| 21 EAC | wt | wt | wt | na |
| 22 EAC | L23Q | wt | wt | na |
| 23 EAC | P76T | wt | wt | na |
| 24 EAC | L23Q | wt | wt | na |
| 38 ESCC | S8R | na | na | na |
| 39 ESCC | G30N | na | na | na |
| 40 ESCC | exon 1 24 bp insert | na | na | na |
| 41 ESCC | S8R | na | na | na |
| 42 ESCC | S8R | na | na | na |
| 43 ESCC | S8R | na | na | na |
| 44 ESCC | codon 18 CA insertion | na | na | na |