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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Mol Cancer Res. 2008 Aug 22;6(9):1499–1506. doi: 10.1158/1541-7786.MCR-07-2130

FIGURE 4. SIRT1 expression is induced by low glucose incubation, increasing cellular resistance to cisplatin.

FIGURE 4

A. KB-3-1 and BEL7404 cells were incubated in medium with various concentrations (4.5, 2, 1 and 0.5 g/L) of glucose for 3 days. Protein extracts were normalized for Western blotting for SIRT1 expression and actin was used as a control. Representative images are shown from experiments performed in triplicate. B. Low glucose incubation increases cellular resistance to cisplatin in parental CP-s KB-3-1 and BEL7404 cells. Cells seeded in plates were treated with cisplatin at various concentrations. Results were expressed as mean (SD). Comparisons of each group were evaluated by one-way analysis of variance (ANOVA). When twice the S.D. was higher than the mean, a non-parameter test was used to evaluate the difference. A significant difference was assumed to exist when P < 0.05. The results shown are the mean of three individual experiments.