Table I.
Effect of additions on the rotational correlation time (τ2) of spin-labeled cytochrome caa31
Preparations | L’’/L | τ2 (µs) |
---|---|---|
(MSL-caa3 × PL) | 0.70 ± 0.01 | 65 ± 2 |
[(MSL-caa3 + bF1F0) × PL] | 1.06 ± 0.01 | 270 ± 1 |
[(MSL-caa3 × PL) + (bF1F0 × PL] | 0.71 ± 0.01 | 70 ± 2 |
[(MSL-caa3 + mF1F0) × PL] | 0.71 ± 0.01 | 70 ± 2 |
[(MSL-caa3 + mbc1) × PL] | 0.70 ± 0.01 | 65 ± 2 |
The molar ratio of cytochrome caa3 to other proteins used was 1:1.
The data presented are averages of three assays conducted on two independent preparations.