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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Eur J Oral Sci. 2008 Aug;116(4):334–340. doi: 10.1111/j.1600-0722.2008.00547.x

Fig. 4.

Fig. 4

Up-regulation of CSF-1 and MCP-1 expression by EMAP-II in the DF cells after EMAP-II was knocked down by siRNA. DF cells were treated with EMAP-II (50 ng/ml) for 24 h after endogenous EMAP-II expression was knocked down. Expression of CSF-1 (A) and MCP-1 (B) were determined using real-time RT-PCR. Control: transfection without siRNA; siRNA: transfection with 2 nM siRNA; siRNA+EMAP-II: transfection with 2 nM siRNA for 24 h, and then treated with 50 ng/ml EMAP-II for 24 h. The experiments were repeated three times and the results were analyzed using SAS software. Knockdown of EMAP-II resulted in reduction of CSF-1 and MCP-1 expression, and the expression of CSF-1 and MCP-1 were increased significantly by EMAP-II treatments (P < 0.01).