Fig. 5.

Chemotaxis assay of human EMAP-II on rat bone marrow mononuclear cells. In Transwells, the control was 600 μl MEM medium loaded in the lower chambers (0 nM) and treatments were MEM plus human EMAP-II (0.1-10 nM). In the upper chamber, 100 μl bone marrow mononuclear cells were loaded. After 2 h, the cells that migrated through the membrane into lower chambers were collected and counted. The migration index was expressed as the ratio of the cell number in lower chambers with EMAP-II to that in lower chambers without EMAP-II. The experiments were repeated four times, and the results were analyzed with SAS software. The human EMAP-II attracted a significant number of cells (P < 0.01) at all concentrations tested.