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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Eur J Oral Sci. 2008 Aug;116(4):334–340. doi: 10.1111/j.1600-0722.2008.00547.x

Fig. 6.

Fig. 6

Reduction in chemotactic activity of the conditioned medium (CM) from the DF cells with EMAP-II antibody added (A) and CM from DF cells with EMAP-II knocked down (B). The chemotaxis assay was performed as in Fig. 5 except that 600 μl CM (Control) or CM plus 10 μg anti-EMAP-II antibody (antibody) or CM from DF cells with EMAP-II knocked down by 10 nM siRNA (siRNA) was loaded in the lower chambers of Transwells. The controls in 6B were CM from the DF cells transfected without siRNA. The migration index was calculated as the ratio of cells migrating into conditioned medium to those migrating into MEM medium. The experiments were repeated four times, and the results were analyzed using paired t-tests with an asterisk (*) denoting a significant difference between treatment pairs. Both anti-EMAP-II antibody (6A) and siRNA targeting EMAP-II mRNA expression (6B) reduced the chemotactic activity of the CM significantly (P < 0.01).