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The active site of the RPE from S. pyogenes (10). Panel A, the interactions of the substrate analog D-xylitol 5-phosphate (green) with the active site. Panel B, the interactions of the phosphate group of D-xylitol 5-phosphate with the phosphate binding motif (Gly 178, Gly 198, and Ser 199) and the active site loop (Gly 146). A 3.2 Å threshold was used for drawing hydrogen bonding and liganding interactions.
