Skip to main content
. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Oncogene. 2007 Feb 12;26(33):4825–4832. doi: 10.1038/sj.onc.1210282

Figure 1. HEI10 depletion inhibits cell proliferation.

Figure 1

A. HEI10 depetion by siRNA was analyzed by RT q-PCR in all experiments described in this study. Results shown are normalized to levels of RNApolR2F. B. Western blot showing HEI10 depleted by two siRNAs, in reference to a-tubulin loading control. C. Immunofluorescence with antibody to HEI10 cells treated with siRNAs to HEI10 and control. Results were consistent between two different siRNAs to HEI10 (second not shown). Note increased number of cells in control panel at day 4 (compare to C). D. Proliferation of cell lines mock-transfected, or transfected with control siRNAs (Scr), or two siRNAs targeting HEI10. E. FACS analysis of cells treated with two HEI10-targeted siRNAs, control siRNAs, or mock-transfected, at 2, 3, 4, and 5 days after treatment. n.d., not determined because of reduced cell numbers. F. Hoechst 33342 staining of nuclei of cells treated with Scr or HEI0-directed siRNAS for 4 days, or with cisplatin (arrows indicate pycnotic nuclei). G. Relative apoptotic index produced by APOPercentage assay at days 3, 4, and 5 after HEI10 depletion. Differences between HEI10- and Scr-depleted cells are not statistically significant.