Table 3.
Apparent Free Energy of unfolding for WT, Q85E, Q180E, and Q85E/Q180E βA3-crystallin.
| Protein | a CM | bΔCM | c Apparent ΔGD0 | d Apparent ΔΔGD0 |
|---|---|---|---|---|
| WTβA3 | 4.5 ±< 0.1 | − | 14 ± 2.0 | − |
| Q85E | 4.2 ±< 0.1 | −0.3 ±< 0.1 | 10 ±0.1 | −4.0 ±2.1 |
| Q180E | 4.1 ±< 0.1 | −0.4 ±< 0.1 | 9.6 ± 0.8 | −4.4 ± 1.9 |
| Q85E/Q180E | 3.7 ±< 0.1 | −0.8 ±< 0.1 | 4.4 ± 0.4 | −9.6 ± 2.4 |
a
The midpoints of unfolding transitions for mutants from extrapolation method.
b
ΔCM=CMmutant-CMwildtype in units of M.
c
The Apparent ΔG 0 (kcal/mol), of unfolding based on urea curves. RT1nK values were calculated for K =fD/fN from the transition region of the urea denaturation curves.
d
Apparent ΔΔGD0=ΔGD0mutant-ΔGD0wild type in units of kcal/mol.