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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Cell Biochem. 2008 Sep 1;105(1):290–300. doi: 10.1002/jcb.21830

Fig. 3.

Fig. 3

The effect of rapamycin on formation of the α4/PP2A-C complex in adult liver. Extract protein was prepared from livers taken from adult rats injected 24 hr previously with DMSO or rapamycin. A: The extracts (80 μg protein per lane) were analyzed by direct immunoblotting for phosphorylated ribosomal protein S6 (P-S6), total S6, PP2A-C and α4. B: Extracts (1 mg protein) were analyzed by immunoprecipitation with α4 antibody, followed by immunoblotting for PP2A-C and α4. The ratio of PP2A-C to α4 in the immunoprecipitates is shown in the bar graph as the mean + 1 standard deviation. *, P<0.05 versus DMSO control.