Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Cell Biochem. 2008 Sep 1;105(1):290–300. doi: 10.1002/jcb.21830

Fig. 8.

Fig. 8

α4/PP2A-C and rapamycin resistance in rat hepatic cell lines. A panel of rapamycin-sensitive (H4-II-E, GN5, 1682-A, WB) and rapamycin-resistant (WB311, GP6, H5D, THC-252) hepatic cells were studied. A: Lysate protein (20 μg) from cells was analyzed by direct immunoblotting for content of α4 and PP2A-C. The latter employed antibodies directed toward the mid-portion (MD-PP2A) and carboxy-terminus (CT-PP2A) of PP2A-C. B: WB and WB311 cells were exposed to rapamycin for times varying from 10 min to 6 hr. Cell lysates were analyzed by immunoprecipitation with α4 antibody followed by immunoblotting for PP2A-C and α4.