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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Steroids. 2008 May 18;73(11):1160–1173. doi: 10.1016/j.steroids.2008.05.003

Figure 1. Functional expression of human mPRα, mPRβ and mPRγ in yeast.

Figure 1

All cells are wild type and are grown in iron-deficient LIM. Medium in panels (A) and (B) contains 0.05% galactose/1.95% raffinose and medium in panel (C) contains 2% galactose. In all boxes, the activity of the FET3 gene is monitored by measuring β-galactosidase activity produced by the FET3-lacZ reporter. (A) All PAQRs are cloned into the pYES260 vector except AdipoR2, which is cloned into pGREG536. White symbols show the effect of progesterone on FET3 in cells carrying either empty expression vector or vectors that express mPRα, mPRβ or mPRγ. Grey symbols show the effect of adiponectin on FET3 in cells carrying either empty expression vector or vectors that express AdipoR1 or AdipoR2. (B) The effect of various steroids on the FET3 gene cells expressing either mPRγ or mPRα from the pYES260 plasmid. (C) The effect of various steroids on FET3 in cells expressing mPRα from the pRS316 plasmid. When data points overlap or are very close to overlapping, combined symbols are used.

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