Figure 5.
Global treatment of neurons with NGF and inhibitors of NGF pathways. Addition of 50 ng/ml NGF to the culture medium increased the Fm/Fc. The effect of 50 ng/ml NGF is significantly diminished by adding 100 μm PI3 kinase inhibitor LY294002 or 10 μm MAPK inhibitor U0126. A combination of LY294992 and U0126 decreased Fm/Fc only as much as U0126 alone. U0126 decreased Fm/Fc in neurons cultured in pyruvate when compared with cells also cultured in pyruvate but not treated with U0126. From left to right, n = 26, 22, 18, 17, 24, and 21 neurons. *p < 0.001. Error bars show SEM.