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HEK293T cells transfected with indicated GFP fusion constructs were treated with lysophosphatidic acid and then RhoA-GTP levels were analyzed by GST-RBD pull down assay followed by anti-RhoA blotting (top). The expression levels of endogenous RhoA (middle) and recombinant GFP-fusion proteins (bottom) were confirmed by immunoblotting with anti-RhoA and anti-GFP antibodies.
