The spinal trigeminal (STr), anterolateral (AL), trigeminal lemniscus (TL) and medial lemniscus (ML) tracts were segmented by placing seeding masks (white asterisks) in lateral medulla regions and implementing waypoints (white asterisks) in lateral midbrain (Subject 6, See also Segmentation 3 in Methods and Materials). The locations of the masks were based on results shown in Figure 3 and also a brainstem anatomical atlas (www.msu.edu/~brains). A. Coronal slices through the brainstem where the STr, AL, TL and ML tracts are present. Red lines in each coronal slice represent the location of the axial slices shown in B. B. Axial slices through 4 sections of the brainstem where STr, AL, TL and ML tracts are located. C. Histological cross sections of the brainstem corresponding to the general location of the axial slices shown in B. Red circles indicate location of the STr, AL, TL and ML in each cross section. (All coronal and axial slices have 1mm slice thickness and in native T1-weighted anatomical space.) Histological cross sections were reprinted with permission from the Michigan State University Brain Biodiversity Bank (www.msu.edu/~brains) and the National Science Foundation.