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GSE-I modulates Cip1/p21 and Kip1/p27 levels in a panel of human CRC cell lines. LoVo, HT29 and SW480 cells were treated with either DMSO alone (control) or varying concentrations of GSE-I (25-100μg/ml) for 12 and 24 h. At the end of these treatments, total cell lysates were prepared and analyzed by western blotting for p21 and p27 levels as detailed in the Materials and Methods. Membranes were stripped and reprobed for tubulin as a loading control.
