Figure 3. Nun induces ribosomal operon transcription in a Fis-dependent fashion.

Strains grown overnight at 30 °C were diluted 1:100 in LB and grown at 30 °C. Four hr later 100 µM IPTG was added where indicated and β-galactosidase (MU) were determined hourly. Strains in panels A and B carry the p1-p2 promoters of rrnE fused to lacZ (Table 1). A) fis⁺; B) fis::767; C) fis⁺, the p2 promoter of rrnB is fused to lacZ - Fis binding sites are deleted.

The above data are an average of two independent experiments.