TABLE 1.
Strains, plasmids and phages used in this study
| Strains, plasmids, and phages | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| N99 | W3102; Wild-type | NIH collection |
| RJ1800 | MG1655 fis::767 | 16 |
| CA8224 | HfrH placUV5- lacZ | Beckwith J. collection |
| AU221 | N99/pAUM | This work |
| AU222 | N99/pAUMNun | “ |
| AU223 | N99/pAUMNunP9A | “ |
| AU227 | N99 lacZXA21(λ 423)/pAUM | “ |
| AU228 | N99 lacZXA21(λ 423)/pAUMNun | “ |
| AU230 | N99 nusAE136K/pAUM | “ |
| AU231 | N99 nusAE136K/pAUMNun | “ |
| AU246 | N99/pAUMNunW108A | “ |
| AU251 | N99 lacZXA21(λ 4253)/pAUM | “ |
| AU252 | N99 lacZXA21(λ 4253)/pAUM Nun | “ |
| AU260 | N99 lacZXA21 lacUV5-lacZ+/pAUM | “ |
| AU261 | N99 lacZXA21 lacUV5-lacZ+/pAUMNun | “ |
| AU265 | N99 lacZXA21(λ 423), fis:767/pAUM | “ |
| AU266 | N99 lacZXA21(λ 423), fis:767/pAUMNun | “ |
| AU269 | N99 lacZXA21(λ HS3120)/pAUM | “ |
| AU270 | N99 lacZXA21(λ HS3120)/pAUMNun | “ |
| AU271 | N99 lacZXA21(λ HS3134)/pAUM | “ |
| AU272 | N99 lacZXA21(λ HS3134)/pAUMNun | “ |
| AU278 | AU221/pBAD30 | “ |
| AU297 | AU221/pBWCRP | “ |
| AU284 | AU223/pBAD30 | “ |
| AU299 | AU223/pBWCRP | “ |
| Plasmids | ||
| pTrc99 | ColE1ori ampR | Amersham Biosciense |
| pTrcNun | pTrc99-nun+ | Our lab collecton |
| pBAD30 | pACYC184 ori ampR | 25 |
| pBAD18 | ColEIori camR | 25 |
| pHY0 | pEXT20-crp | 26 |
| pAUM | pTrc99, ampS, camR | This work |
| pAUMNun | pAUM-nun+ | “ |
| pAUMNunP9A | pAUM-nunP9A | “ |
| pAUMNunW108A | pAUM-nunW108A | “ |
| pBWCRP | pBAD30-crp | “ |
| Phages | ||
| λ 423 | λcI857 rrnEP1P2 | 27 |
| λ4253 | λcI857 rrnBP2 | 28 |
| λHS3120 | λ pmalE | H. Shuman collection |
| λHS3134 | λ pmalP | H. Shuman collection |