Table 2.
nusAE136K and nunW108A suppress Nun toxicity
Strain | Host mutation | Plasmid | Growth rate(hr−1)a |
---|---|---|---|
AU221 | nusA+ | vector | .71 +/− .01 |
AU222 | nusA+ | nun + | .30 +/− .01 |
AU230 | nusAE136K | vector | .70 +/− .01 |
AU231 | nusAE136K | nun + | .53 +/− .04 |
AU246 | nusA+ | nunW108A | .68 +/− .02 |
Strains were grown overnight and diluted 1:100 into 50µM IPTG.
The initial OD600 was subtracted from the OD600 at 6 hr and divided by 6.