Table 4.
Nun inhibits malE and malP promoters
| Strain | lacZ fusion | nun | β-galactosidase | % inhibition |
|---|---|---|---|---|
| AU269 | pmalE | − | 2933 +/− 106 | - |
| AU269 | pmalE | + | 565 +/− 88 | 85 |
| AU270 | pmalP | − | 662 +/− 72 | - |
| AU270 | pmalP | + | < 1 | >99 |
Overnight cultures were diluted 1:100, grown for 1 hr at 37 °C, and then brought to 100µM IPTG and 0.2% maltose to induce nun and mal promoters, respectively. β-galactosidase activity (MU) was determined after an additional 3 hr at 37 °C.
The above data are an average of two independent experiments.