Table 5.
Crp overproduction suppresses Nun toxicity and restores lac transcription.
| Strain | Nun | Crp | β-galactosidase | Growth rate(hr−1)a |
|---|---|---|---|---|
| AU278 | − | − | 425 +/− 70 | .68 +/− .01 |
| AU297 | − | + | 505 +/− 88 | .67 +/− .02 |
| AU284 | + | − | 146 +/− 10 | .11 +/− .01 |
| AU299 | + | + | 502 +/− 90 | .39 +/− .02 |
Overnight cultures were diluted 1:100 in LB +ampicillin (50µg/ml) + chloramphenicol (50µg/ml), grown for 1 hr at 37 °C, and treated with 100 µM IPTG and 0.02%arabinose to induce nun and crp, respectively. β-galactosidase activity (MU)) was determined after an additional 5 hr at 37 °C. The above data are an average of two independent experiments.
a
Growth rate was determined as in Table 2.