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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Biomol NMR. 2008 Sep 12;42(2):99–109. doi: 10.1007/s10858-008-9267-x

Figure 1.

Figure 1

800 MHz 1H-15N shift correlation of tRNAVal. (A) Part of the imino region of the superimposed 2D 15N-1H TROSY-HSQC (blue) and regular HSQC (red) correlation spectra of tRNAVal recorded at 800 MHz, 28 °C, showing correlations for the imino groups of G nucleotides. (B) Expanded cross peak for the TROSY imino correlation of U64, which forms a wobble base pair with G50 and experiences extensive homonuclear 1H-1H RDC with adjacent protons. 15N-1H/1H-1H dipolar cross correlated relaxation results in asymmetry of the unresolved 1H-1H multiplet.

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