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. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: J Endocrinol. 2008 Aug 13;199(2):333–341. doi: 10.1677/JOE-08-0306

Lifelong running results in reduced levels of lipid peroxidation, and protein carbonylation and nitration in the testes. Levels of H2O2 (A), isoprostanes (B), nitrotyrosine (C) and protein carbonyls (D) were measured in testicular samples sedentary mice and runners. Values are the mean and SEM (n = 6 mice per group) *p< 0.01. Proteins isolated from testis of sedentary mice and runners were used to measure levels of antioxidant enzymes (SOD, Cu/Zn-superoxide dismutase; HO-1, heme oxygenase; GPX, glutathione peroxidase) and γ-H2AX (E and F) and proteins modified by the lipid peroxidation product 4-hydroxynonenal (HNE) (G and H). Values are the mean and SEM (n = 6 mice per group) *p<0.05. ***p<0.001, **p<0.01. I. mRNA levels of SOD1, SOD2, GPX-1 and GPX-4 in sedentary mice compared to runners. Values are the mean and SEM (n = 6 mice per group). *p<0.001.

Lifelong running results in reduced levels of lipid peroxidation, and protein carbonylation and nitration in the testes. Levels of H2O2 (A), isoprostanes (B), nitrotyrosine (C) and protein carbonyls (D) were measured in testicular samples sedentary mice and runners. Values are the mean and SEM (n = 6 mice per group) *p< 0.01. Proteins isolated from testis of sedentary mice and runners were used to measure levels of antioxidant enzymes (SOD, Cu/Zn-superoxide dismutase; HO-1, heme oxygenase; GPX, glutathione peroxidase) and γ-H2AX (E and F) and proteins modified by the lipid peroxidation product 4-hydroxynonenal (HNE) (G and H). Values are the mean and SEM (n = 6 mice per group) *p<0.05. ***p<0.001, **p<0.01. I. mRNA levels of SOD1, SOD2, GPX-1 and GPX-4 in sedentary mice compared to runners. Values are the mean and SEM (n = 6 mice per group). *p<0.001.