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. Author manuscript; available in PMC: 2009 Feb 14.
Published in final edited form as: Cell Host Microbe. 2008 Aug 14;4(2):111–122. doi: 10.1016/j.chom.2008.06.007

Figure 2. A dominant inhibitory activity restricts PB2 function in human cells.

Figure 2

(A) Experimental strategy for formation of heterokaryons by expression of the fusogenic VSV-G protein. Heterokaryon formation between 293T cells expressing GFP and DF1 cells expressing RFP was monitored by the detection of dual-fluorescent cells (arrowheads). (B) Polymerase activity in heterokaryons expressing NP, PB1, PA, and no PB2, WT, or K627E PB2-TAP was determined by luciferase assay. Protein expression was monitored by western blot. n=3 +/− standard deviation.