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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: J Med Chem. 2006 Mar 23;49(6):1991–1999. doi: 10.1021/jm051049o

Table 1.

19F chemical shifts + and hydrolytic rates *

Reporters 4 12 13 GFPOL
δF (substrate) −3.02 −2.85 −2.14 −3.22
δF (product) −12.37 −12.16 −11.22 −11.21
ΔδF 9.35 9.31 9.08 7.99
ν(μmol/min/unit) 34.0 35.0 38.0 4.3
+

ppm with respect to sodium trifluoroacetate.

*

β-gal (E801A) added at 37°C in PBS (0.1 M, pH=7.4).