Table 1.
Reporters | 4 | 12 | 13 | GFPOL |
---|---|---|---|---|
δF (substrate) | −3.02 | −2.85 | −2.14 | −3.22 |
δF (product) | −12.37 | −12.16 | −11.22 | −11.21 |
ΔδF | 9.35 | 9.31 | 9.08 | 7.99 |
ν(μmol/min/unit) | 34.0 | 35.0 | 38.0 | 4.3 |
ppm with respect to sodium trifluoroacetate.
β-gal (E801A) added at 37°C in PBS (0.1 M, pH=7.4).