Figure 3.

KiSS-1 activates MSDB vGluT2-GFP neurons in prepubertal and postpubertal male and female mice. a1–a3, Current-clamp recordings show excitatory effects of 1 nm to 1 μm KiSS-1 in male and female mice of different ages. vGluT2-GFP mice used in this study become reproductively active at postnatal day 30. Note the bursting activity in a2. KiSS-1-activated vGluT2-GFP neurons often displayed bursting activity both in the absence and presence of KiSS-1. b, Bar chart shows that the number of cells that responded to different concentrations of KiSS-1 in prepubertal versus pubertal mice did not vary significantly, albeit in both groups, ∼65% of neurons responded to 1 nm KiSS-1 with an excitation, whereas 100% of neurons responded to 100 nm KiSS-1. c, Depolarization in millivolts in response to 1 and 10 nm KiSS-1 in the prepubertal versus postpubertal mice. Note the insignificant difference (n.s.). Error bars indicate SEM.