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. Author manuscript; available in PMC: 2009 Apr 16.
Published in final edited form as: Cell Host Microbe. 2008 Oct 16;4(4):337–349. doi: 10.1016/j.chom.2008.09.009

Figure 3. Th17 Cell Differentiation during Ontogeny.

Figure 3

Lamina propria lymphocytes were isolated from the small intestines of Rorγt gfp/+ mice at the indicated ages. For day 4 and day 16 studies, small intestines from 3-4 pups from the same litter were combined in order to obtain sufficient cell numbers for analysis. For day 25 and 33, plots represent individual mice. Top panels — surface staining and RORγt-GFP analysis; bottom panels — intracellular staining for IL-17 after 4 hour stimulation with PMA/Ionomycin in the presence of GolgiPlug (BD). Data are representative of two independent experiments with 2-3 samples/mice in each. Plots are gated on lymphocytes.