Fig. 2.
Effects of altered FoxM1 expression on VEGF expression in human glioma cell lines. A, Upregulation of VEGF mRNA and protein expression by FoxM1B overexpression. FoxM1 and VEGF expression levels in parental, control-vector transfected, and FoxM1 expression-vector transfected SW1783 and Hs683 cells were determined by Northern blot analyses (A1), and Western blot analyses (A2). B, Downregulation of VEGF mRNA and protein expression by knockdown of FoxM1 expression. FoxM1 and VEGF expression in U-87MG and HFU-251MG cells transfected with FoxM1-siRNA or control siRNA were determined by real-time RT-PCR (B1) and Western blot analyses (B2).