Figure 2.

Human Goodpasture patient sera (GPS) inhibit antigen binding by mouse monoclonal antibody reactive with anti-alpha3(IV) NC1 collagen. The concentration (5 microg/ml) of mouse mAb that gave 50% maximal binding to antigen was preincubated with varying dilutions of inhibitor (human serum, shown as reciprocal dilution on x-axis). Results using serum from three different patients and human serum from a healthy control are shown.