Figure 5.

Anti-alpha3(IV)NC1 reactivity of Tg mouse serum. A) Scatterplot of serum reactivity. Mouse serum diluted 1/20 was incubated on microwells coated with alpha3(IV)NC1 collagen diluted in 6 M guanidine, or on wells coated with diluent alone, and detected with labeled anti-allotype IgM-a-specific second step reagent. OD₄₀₅ represents value for binding to antigen minus diluent. Values for individual transgenic (Tg) and non-Tg mice are indicated. B) Human GPS patient serum inhibition of antigen-binding by Tg mouse serum. The dilution of Tg mouse serum that gave 50% maximal binding to alpha3(IV) NC1 collagen was incubated with varying dilutions of inhibitor (GPS patient serum, shown as reciprocal dilution on x-axis). Shown are results using serum from two Tg mice, Tg1 and Tg2, and human serum from two GPS patients, GPS1 and GPS2, and a healthy control (normal serum).