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. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2008 Oct 21;47(46):12118–12125. doi: 10.1021/bi8011548

Figure 2. Plots of the fraction of DNA (CII5T) bound vs the concentration of enzyme. WT (●), I260Q (○).

Figure 2

The Kaleidagraph graphics program was used to plot the data, and the data were fit as described in Experimental Procedures to obtain the KD. KD for WT is 9.3 ±1.5 nM and for I260Q=1.8±0.2nM.