Skip to main content
. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2008 Oct 21;47(46):12118–12125. doi: 10.1021/bi8011548

Figure 3. Single turnover experiments of correctly paired CII5T substrate using rapid quench apparatus.

Figure 3

A. Incorporation of dCTP opposite template G (substrate CII5T) for I260Q at 37°C. A preincubated solution containing 750nM enzyme and 50nM substrate was mixed with 10mM MgCl2 and 25(○),50(●),100(□),.200 (△) and 300 (■) μM dCTP. The reactions were quenched by EDTA at different time points, and the products were resolved by denaturing sequencing gel electrophoresis. The data were fit to the single exponential equation to obtain the kobs. B. The secondary kinetic plot of kobs versus the dCTP concentration for I260Q (●). The solid line represents the best fit of the data to the hyperbolic equation. The kpol is 10.8 sec−1 and Kd is 165.8 uM.