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. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2008 Oct 21;47(46):12118–12125. doi: 10.1021/bi8011548

Figure 4. I260Q extends mispaired primer-termini with incorrect bases.

Figure 4

A missing base primer extension assay was carried out as described in Materials and Methods. The substrate employed was CII5T primer-template that contains a mispaired (A:A) at the 3′end of gthe 5 nucleotide gap. 50nM of mispaired substrate was incubated with either 750nM WT or I260Q protein. Reactions were conducted in presence of only one type of 100uM dNTP (dATP, dTTP or dGTP) at 37°C for different time intervals (4–30 min). A. A phosphorimager representation of sequencing gel of mispair extension by incorrect dNTP. The dNTP used was indicated at the bottom of the gel. B. A quantitative representation of mispair extension by WT and I260Q. The amount of mispair extension by each dNTP at 30 minutes was plotted against the respective dNTP. The filled bar represents WT and dotted bar is for I260Q.