The ehIFRPF motif is an evolutionarily novel, conserved insect-specific GRO-interaction motif. Location of the ehIFRPF motif relative to the previously characterized eh1 and eh2 sequence motifs, which physically interact with GRO and EXD, respectively. The D. melanogaster sequence of partially conserved peptide motifs between the eh1 and eh2 motifs are shown. ..., non-conserved (dipteran/lepidopteran clade) bases not shown. No function is known for the motif whose sequence in D. melanogaster is “LGSLCKAVSQIG”. Possible ehIFRPF motifs with one mismatch (lowercase) are also shown for T. castaneum, P. americana, and S. gregaria. No putative ehIFRPF motifs that match the consensus ψΩ(K/R)PΩ (or contain a single mismatch) were found in any other EN/INV homologs (see MATERIALS AND METHODS for organisms and sequences searched).