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. Author manuscript; available in PMC: 2009 May 13.
Published in final edited form as: Nature. 2008 Oct 5;456(7219):269–273. doi: 10.1038/nature07349

Figure 1. Sequential activation of CRTC2 and FOXO1 during fasting.

Figure 1

a, Ad-CRE-luc activity (top) and CRTC2 protein amounts (bottom) in mice fasted for 6 or 24 hours. Intra-peritoneal glucagon injection indicated. b, and c, Effect of 6 or 18 hour fasting on Ad-G6Pase-luc activity (b), G6Pase mRNA amounts (c), and blood glucose concentrations (c) in mice infected with Ad-CRTC2i, Ad-FOXO1i, or (USi) control virus (n=4, (*) P< .05; (**) P< .02; (***) P< .01). d, Top, activities of wild-type or mutant Ad-G6Pase-luc reporters defective in CREB (CREmut) or FOXO1 (IREmut) binding. Mice were fasted for 6 or 18 hours as indicated (n=4, *;P<.05). Bottom, chromatin immunoprecipitation (ChIP) assay showing binding of myc-tagged FOXO1 or Flag-epitope tagged CRTC2 to the G6Pase promoter in HepG2 hepatocytes exposed to FSK for 6 or 18 hours. For panels b, c, d, data are means ± s.e.m.