IFN-γ production by iNKT cells in response to CpG stimulated DCs. A, WT DCs were stimulated with CpG ODN or non-activating CpG ODN (Non CpG), co-cultured with purified splenic iNKT cells, and cytokine production was measured by ELISA. B, Purified iNKT cells were co-cultured with CpG or non-CpG ODN stimulated DCs derived from the indicated mouse strains, or DCs from CD1d-/- or WT mice in the presence of anti-CD1d blocking mAb. Cytokine production was measured in the supernatant by ELISA. Shown is a representative experiment out of 5 performed. Results represent the mean of 1 experiment with 3 replicate cultures measured in triplicate for ELISA. Error bars, SEM (n=9 for each set of DCs).