Figure 3. The zonulin peptide inhibitor AT-1001 reduced small intestinal permeability by 8 weeks. (A) Mannitol flux was measured in Ussing chambers at 8 weeks of age and was increased in the interleukin 10 gene-deficient (IL10⁻/⁻) mice (p<0.05). This increase was not observed in animals treated with AT-1001 (n = 4–8). (B) The electrical resistance was reduced in the IL10^−/− mice only (p<0.01). AT-1001 treatment prevented this reduction (n = 5–8). WT, wild-type mice.